Lipid Research Division

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  • 1.  A paper you remember!

    Posted 27 days ago

    Hi All! 

    Just a fun little question on a busy Thursday Morning!

    What is that one paper/field of research/scientist/book, etc that made a long lasting impression on you? (can list multiple :) ofcourse! Science is so widespread and cool!)

    Happy Thursday! 



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    Udipta Bohara
    Graduate Student
    Virginia Tech
    Blacksburg VA
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  • 2.  RE: A paper you remember!

    Posted 26 days ago

    Oh, that's easy.  It is from Kentaro Hanada. "Genetic evidence for ATP-dependent endoplasmic reticulum-to-Golgi apparatus trafficking of ceramide for sphingomyelin synthesis in Chinese hamster ovary cells"  1999. J Cell Bio  144:63.

    Before Crispr, sequencing of the human genome, before RNA-Seq, before you could order an expression plasmid for any protein you were interested in, before siRNA was in common use.  Dr. Hanada was looking for sphingomyelin synthase. He used a clever mutational strategy utilizing a toxin that binds to sphingomyelin and kills cells, to identify mutations in  that abolished sphingomyelin synthesis. But he realized that the sphingomyelin synthase itself had full activity and was imaginative and broad minded enough to figure out that it was transport of ceramide from the ER to the Golgi that was affected.  Amazing synthesis of biochemistry and cell biology. He went on to clone the protein, we now know as CERT. 



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    Brian Wattenberg
    Professor
    Virginia Commonwealth University
    Richmond VA
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    Original Message


  • 3.  RE: A paper you remember!

    Posted 26 days ago

    There are many.  One really stuck with me as being a fascinating piece of informed food for thought.  I happened to run across this paper about 5 yrs ago:  "Evolutionary Origin of Cardiac Malformations", by Helen Taussig,  Journal of American College of Cardiology, Oct 1988, p. 1079.   The author, a prominent pediatric cardiologist at the time, examines heart malformations in birds and their similar counterparts in humans. I don't know how well this line of thought has stood up in subsequent years.  No matter, it's fascinating reading. 



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    Blaise Arena
    retiree
    none
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    Original Message


  • 4.  RE: A paper you remember!

    Posted 26 days ago

    This is easy.  It's the paper by Howard Dintzis in 1961 that showed proteins were synthesized from the N-terminus to the C-terminus without the use of any kits (there weren't any) (H. M. 1961. Assembly of the peptide chains of hemoglobin. Proc. Nat. Acad. Sci. U.S.A. 47:247-261).   It's a classic and a great example of how someone thought about an important problem and came up with a creative way to address it.  Before he did this experiment there had been a controversy as to the direction of protein synthesis.  He examined the time-dependent incorporation of radiolabeled leucine (both 3H and 14C) into hemoglobin synthesized in a reticulocyte lysate and a carboxypeptidase and definitively showed the direction was from the N to the C terminus. 

     

    Daniel M. Raben, PhD

    Professor Emeritus

    Departments of Biological Chemistry, Physiology, Oncology

    Emeritus Associate Director of the MSTProgram

    The Johns Hopkins University School of Medicine

    725 North Wolfe St.

    Baltimore, MD 21205

    Tele: 410-955-1289

     




    Original Message


  • 5.  RE: A paper you remember!

    Posted 26 days ago

    Maybe a little too young version of the answer, but I was impressed by Bruno Mesmin et al., 2013, A Four-Step Cycle Driven by PI(4)P Hydrolysis Directs Sterol/PI(4)P Exchange by the ER-Golgi Tether OSBP. They proposed a remarkable mechanism of lipid transfer, full-circling the initiation and stop of lipid transfer in one paper. Obviously there had been a substantial amount of previous research before this lipid trafficking protein, including Brian's recommendation about CERT. But for personal reasons, I discussed this paper in my journal club during my PhD, and this paper showed me what I would like to do as a researcher, and I persuaded myself to do post-doc research in lipid biology. Good or bad, impactful or not, your judge!



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    Yeongho Kim
    Associate Research Scientist
    Yale University
    New Haven CT
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    Original Message


  • 6.  RE: A paper you remember!

    Posted 25 days ago

    OMG!  Myles Brown estrogen receptor dynamics paper by ChIP: https://pubmed.ncbi.nlm.nih.gov/11136970/ in 2000. Also, it seemed like David Allis put out a Cell/Science/Nature paper once a month around that time, all incredibly intriguing!  As a postdoc Sarah Spiegel's 2009 Science paper on nuclear S1P was inspiring, as was Gabe Schaaf and Vytas Bankaitis 2007 Sec14 paper, and then of course I found out about the older nuclear phosphoinositide papers from Nullin Divecha, Lucio Cocco and Robin Irvine. OK I'll stop.



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    Raymond Blind
    Assistant Professor
    Vanderbilt University School of Medicine
    Nashville TN
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    Original Message


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